中国食品添加剂Issue(6)：122-127,6.
响应面法优化茶多酚生产工艺及其体外抗氧化活性的研究
Study on tea polyphenols manufacturing techniques by response surface method and its antioxidant properties in vitro
陈丽 1蔡利燕 2蒲凌云 1程远欣 2田洪2
作者信息
- 1. 河北省天然色素工程技术研究中心,邯郸057250
- 2. 晨光生物科技集团股份有限公司,邯郸057250
- 折叠
摘要
Abstract
[Objective] In this paper, manufacturing techniques of tea polyphenols were studied by single factor test and response surface method. The total tea polyphenols and yield were the indexes. [ Method] The effects of ratio of concentrated solution and ethyl acetate, ethyl acetate extraction times and ratio of concentrated solution and phosphoric acid solution on the polyphenols content and yield were studied. The antioxidation activities of TP in vitro were investigated. [ Results] The results showed that the optimized manufacturing techniques of tea polyphenols were as follows: ratio of concentrated solution and ethyl acetate at 1 : 1. 60 (V/V) , ethyl acetate extraction twice and ratio of concentrated solution and phosphoric acid solution was at 1. 62 (V/V). The total tea polyphenols was 99. 32% using the optimized technology. The yield was 80. 96%. The IC50, value of tea polyphenols on DPPH? is 1. 10mg/mL. [ Conclusion] The manufacturing technique of tea polyphenols is an efficient method with high total tea polyphenols contend and yield.关键词
茶多酚/单因素实验/响应面法/抗氧化/多酚含量Key words
tea polyphenols/ single factor test/ response surface method/ antioxidant/ polyphenols content分类
轻工纺织引用本文复制引用
陈丽,蔡利燕,蒲凌云,程远欣,田洪..响应面法优化茶多酚生产工艺及其体外抗氧化活性的研究[J].中国食品添加剂,2012,(6):122-127,6.
