中国食品添加剂Issue(6)：134-141,8.
超细粉碎对枸杞多糖得率、结构及抗氧化活性的影响
Effects of superfine grinding on yield rate, structure and antioxidant activities of polysaccharides from Lycium barbarum L.
摘要
Abstract
The superfine grinding assistant extraction conditions was established and the effects of superfine grinding treatment on the structure and antioxidant activities of Lycium barbarum polysaccharides (LBP) were studied in this paper. Based on single factor tests, the superfine grinding assistant extraction conditions were optimized by Box -Benhnken design and response surface methodology ( RSM). The structure characteristics and antioxidant activities of Lycium barbarum polysaccharides ( LBP) were comparatively investigated by gel permeation chromatography, atomic force microscopy (AFM) , DPPH and ABTS free radical assay. Results: The optimum extraction conditions were as follows : ratio of material solvent 1:6, extraction temperature 83. 8 C, extraction time 95 min and extracting 3 times. The yield rate of polysaccharide extracted by superfine grinding was 4. 00% ±0. 13%. Compared with conventional extraction methods, the yield rate increased by 15.61%. Superfine grinding treatment could decrease the average molecular weight, the spherical particle' s heights and the spherical particle' s diameter of LBP. Furthermore, it showed stronger antioxidant activities compared with conventional extraction methods in DPPH and ABTS free radical assay. In conclusion, superfine grinding technique could effectively increase the extraction rates of LBP, decrease the average molecular weights and enhance the antioxidant activities of LBP.关键词
枸杞多糖/得率/结构/抗氧化活性/超细粉碎Key words
lycium barbarum polysaccharides/ yield rate/ structure/ antioxidant activities/ superfine grinding分类
轻工纺织引用本文复制引用
张民,王芳,唐秀丽,董家美,吴娜..超细粉碎对枸杞多糖得率、结构及抗氧化活性的影响[J].中国食品添加剂,2012,(6):134-141,8.基金项目
国家自然科学基金项目(30972042) （30972042）
"十二五"国家科技支撑计划项目(2012BAD33B08). （2012BAD33B08）
