中国食品添加剂Issue(6)：237-241,5.
柱前衍生化-HPLC测定罗非鱼中氨基酸的含量
Detection and quantification of amino acids of tilapia by reversed-phase high performance liquid chromatography
摘要
Abstract
Cuangxi is tilapia main producing region. The determination of tilapia atnino acids have an important role in achieving the high value for Tilapia industry. The determination of Tilapia amino acids was carried out by pre - column derivatization with o - phthalaldehyde ( OPA) and 9 - flu - orenylmethylchloroformate ( FMOC ) and RP - HPLC. HPLC separation of the derivatives was performed using an XDB - C18 column with NaAc buffer, methanol and aceto -nitrile mobile phase system with DAD detector and at 35 C in 40 min. Using this method, Amino acids component of Tilapia were determined. The results showed that there were at least 18 kinds of amino acids in proteins of Tilapia in which the total quantity of 8 essential amino acids ( EAA) up to 49.96% . It can be concluded that the protein of Tilapia is a good protein resource.关键词
柱前衍生化/HPLC/罗非鱼/氨基酸Key words
pre - column derivatization/ HPLC/ Tilapia/ amino acids分类
轻工纺织引用本文复制引用
周利琴,周敬豪,徐存华,孙建华,廖丹葵..柱前衍生化-HPLC测定罗非鱼中氨基酸的含量[J].中国食品添加剂,2012,(6):237-241,5.基金项目
广西科技攻关资助项目(桂科攻10123008-207) （桂科攻10123008-207）
研究生创新计划(105931001-006). （105931001-006）
