中国食品学报2012，Vol.12Issue(11)：24-31,8.
南瓜多糖的分离、纯化及抗氧化活性研究
Study on the Purification, Seperation and Antioxidant Activity of Polysaccharides from Pumpkin
摘要
Abstract
Ion exchange chromatography media DEAE-Sepharose Fast Flow and Gel filtration chromatography media Sephacryl S-300 HR were employed for purification and separation of pumpkin polysaccharides, the radical-scavenging activities and antioxidant activity of fraction P11、 P12、 P21 were determined. The results had shown that the pumpkin crude polysaccharides, P12 and P21 exhibited DPPH radical scavenging activity with 1C50 value 5.496 mg/mL, 8.908 mg/mL, 3.153mg/mL, respectively; pumpkin crude Polysaccharides, P11, P12 and P21 exhibited hydroxyl radical-scavenging activity with IC50 value 4.251 mg/mL, 1.191 mg/mL, 7.655 mg/mL, 5.221 mg/mL, respectively; the FRAP value of pumpkin crude Polysaccharides, P11, P12 and P21 were 0.182mmol/g、 0.062 mmol/g、 0.082 mmol/g、 0.400mmol/g, respectively.关键词
南瓜多糖/纯化/清除自由基能力/抗氧化活性Key words
pumpkin polysaccharide/ purification/ radical-sacvenging activity/ antioxident activity引用本文复制引用
刁文超,吴昊,杨绍兰,张宏斌,王成荣..南瓜多糖的分离、纯化及抗氧化活性研究[J].中国食品学报,2012,12(11):24-31,8.基金项目
山东省现代蔬菜产业技术体系 （）
