中国食品学报2012，Vol.12Issue(11)：142-147,6.
柱前衍生HPLC同时测定鱼中多种生物胺及其变化规律
Biogenic Amines in Fish Determined by HPLC with Pre-column Dansylation
摘要
Abstract
Objective: Seven biogenic amines (including tryptaraine, putrescine, cadaverine, hisamine, tyramine, spermidine and spermine) in fish were simultaneously determined by high-performance liquid chromatograph (HPLC), and the changes of biogenic amines of fish stored at different conditions were investigated. Method: After being extracted with 5% TCA from the aquatic products, the biogenic amines were derived by dansyl chloride before the separation with a C18 column (4.6 mm I.D. xl50 mm). The biogenic amines were eluted using a mixture of water and acetonitrile at a gradient elution program, and were detected at 254 nm. The changes of biogenic amines in the two species of Pseudosci-aena crocea and Dentex tumifrons separately stored at -20 ℃, 4 ℃ and 25 ℃ for different time were measured. Results: Seven biogenic amines could well be separated within 20 min. A good linear response of biogenic amines ranged from 0.1 to 20 mg/L with γ>0.999. The average recoveries tested by adding standards ranged from 73% to 101% for all amines with RSD from 5.9% to 20.1%. Lower levels of biogenic amines (ranged from 3.95 to 23.63 mg/kg) were found in the seven species of measured fresh sea-fish except for Sinocyclocheilus grahami with the value of 170.8 mg/kg. Results also showed that the amounts of total biogenic amines in the two species of Pseudosciaena crocea and Dentex tumifrons stored at -20 ℃ did not significantly change(P<0.05), while, their values increased when the fish were stored at both 4 ℃ and 25 ℃. In addition, the contents of four biogenic amines including putrescine, cadaverine, hisamine and tyramine significantly increased with the increase of storage time and temperature. Conclusion; The formation of biogenic amines can be efficiently controlled by lower temperature. Putrescine, cadaverine, hisamine and tyramine might be used as the potential freshness indexes for fish.关键词
鱼/生物胺/高效液相色谱/丹磺酰氯Key words
fish/ biogenic amines/ high-performance liquid chromatograph/ dansyl chloride引用本文复制引用
胡月,黄志勇..柱前衍生HPLC同时测定鱼中多种生物胺及其变化规律[J].中国食品学报,2012,12(11):142-147,6.基金项目
国家自然科学基金项目(40771185) （40771185）
福建省基金项目(2012J01046) （2012J01046）
福建省科技重点项目(2012Y0052) （2012Y0052）
