中国食品学报2012，Vol.12Issue(11)：148-153,6.
抗β-葡萄糖苷酸酶(β-GUS)兔单克隆抗体的制备和鉴定
Preparation and Identification of Anti-β-glucuronidase (β-GUS) Protein Rabbit Monoclonal Antibodies
摘要
Abstract
In this research, anti-(3-GUS monoclonal antibodies (McAb) of rabbits were obtained after several procedures. These procedures included injecting the β-GUS protein into rabbits, cells fusion, hybridoma cell screening, plas-mid DNA recombination, monoclonal antibody purity and so on. After that, several relation characters were detected by indirect ELISA, the results showed that the affinity constant was 2.13×109L/mol, the titer of McAb was about 64 000 and the detection limit of β-GUS protein by indirect ELISA was about 50 ng/mL. This paper lays material foundation for ELISA kits which can detect β-GUS qualitatively or quantitatively.关键词
β-葡萄糖苷酸酶(β-GUS)/兔单克隆抗体/间接ELISA/免疫Key words
β-glucuronidase/ monoclonal antibodies/ indirect ELISA/ immune引用本文复制引用
沈金儿,倪庚,郑晓冬..抗β-葡萄糖苷酸酶(β-GUS)兔单克隆抗体的制备和鉴定[J].中国食品学报,2012,12(11):148-153,6.基金项目
国家转基因生物新品种培育科技重大专项(2009ZX08012-010B) （2009ZX08012-010B）
