中国食品学报2013，Vol.13Issue(1)：19-28,10.
1种选择性富集沙门氏菌、志贺氏菌、金黄色葡萄球菌和副溶血性弧菌共增菌培养基SSSV研究
SSSV, a Selective Enrichment Broth for Simultaneous Growth of Salmonella, Shigella,Staphylococcus Aureus and Vibrio Parahaemolyticus
摘要
Abstract
A selective enrichment broth (SSSV) was formulated to allow simultaneous growth of Salmonella spp., Shigella spp., Staphylococcus aureus, and Vibrio parahaemolyticus in order to achieve rapid enrichment of these four food-borne pathogenic bacteria in aquatic products. Appropriate additives including accelerants, inhibitors and nutrient substance based on Buffered Peptone Water (BPW) were selected. The optimized ingredients and proportions of additives were determined by the single factor experiment and orthogonal test. The selectivity of SSSV and growth properties of four target pathogens in SSSV were evaluated. The resuscitative ability of SSSV to cold-stressed target pathogens in aquatic products at cold and freezing storage conditions was assessed by artificial contamination. A selective broth, SSSV, was obtained to enrich stimulateously the growth of four pathogens. SSSV broth inhibited some non-target competitive mi-croflora. The enrichment effect of SSSV for four target bacterial was superior than Nutrient Broth (NB), but was inferior to the selective enrichment broths recommended by National Standards. The number of pathogens mixing with different concentration grown form 104 to 107 CFU/mL in the SSSV broth at 37 ℃ for 7 h. The population of pathogens in the peeled shrimp, cuttlefish rolls and fish balls sample inoculated artificially and stored at 4 ℃ and -20 ℃ increased form 105 to 106 CFU/mL using SSSV enrichment at 37 ℃ for 8 h and 12 h, respectively. SSSV broth could recover and resuscitate these four pathogens suffered cold stress. Therefore, it was indicated that SSSV broth can be applied to the pre-enrichment of the test of Salmonella, Shigella, Staphylococcus aureus and Vibrio parahaemolyticus in aquatic products, and would accelerate the test time and improve higher stability of detecting rate and accuracy combined with multiple PCR.关键词
共增菌培养基/沙门氏菌/志贺氏菌/金黄色葡萄球菌/副溶血性弧菌Key words
enrichment broth/ Salmonella/ Shigella/ Staphylococcus aureus/ Vibrio parahaemolyticus引用本文复制引用
翁思聪,朱军莉,冯立芳,励建荣..1种选择性富集沙门氏菌、志贺氏菌、金黄色葡萄球菌和副溶血性弧菌共增菌培养基SSSV研究[J].中国食品学报,2013,13(1):19-28,10.基金项目
国家科技支撑计划(2012BAD29B06) （2012BAD29B06）
浙江省科技厅优先主题项目(2009C03017-5) （2009C03017-5）
