中国食品学报2013，Vol.13Issue(1)：188-194,7.
RP-HPLC法测定桑叶中1-脱氧野尻霉素的含量
Determination of DNJ from Mulberry Leaves by RP-HPLC
摘要
Abstract
Objective:To determine DNJ from mulberry leaves by RP-HPLC with precolumn derivatization. Methods:The separation was performed on a Agela odyssil C18 column(250 mm×4.6 mm, 5 μa), with a mobile phase of acetoni-trile-0.1% acetic acid (40:60). The flow rate was l.0 mL/min and the detection wavelength were set at 254 nm with column temperature at 30 ℃. Results:The linear range of DNJ were from 1.158×l0-3~2.5 mg/mL(R2=0.9998) with recoveries of 100.9%. Conclusion:The method is simple, accurate and responsible for the determination of DNJ from mulberry leaves.关键词
桑叶/DNJ/反相高效液相色谱/衍生化Key words
mulberry leaves/ DNJ/ RP-HPLC/ derivatization引用本文复制引用
郑晓静,伍金娥,何计国,高月..RP-HPLC法测定桑叶中1-脱氧野尻霉素的含量[J].中国食品学报,2013,13(1):188-194,7.基金项目
"十二五"国家科技支撑计划项目(NC2010KB0248) （NC2010KB0248）
