食品工业科技2012,Vol.33Issue(23):117-121,5.
交联壳聚糖对牛血清蛋白的吸附及释放行为
Adsorption and release behavior of BSA by cross-linked chitosan
摘要
Abstract
Using biodegradable chitosan as material, the crosslinked chitosan beads were prepared by crosslinking chitosan with both sodium polyphosphate and epichlorohydrin via ionic and covalent bonds. The adsorption and desorption of bovine serum albumin(BSA) onto cross-linked chitosan were investigated.The effects of initial concentration of BSA, cross-linked chitosan dosage, pH on the adsorption were evaluated, and the adsorption conditions were optimized by orthogonal experiment. The analysis of kinetics for adsorption was preformed.The results showed that the maximum adsorption rate for BSA was 88.7% under the optimum conditions. The adsorption phenomena appeared to follow the pseudo- second order kinetics equation and Freundlich adsorption equation.The cross-linked chitosan carrying BSA could be reused after elution easily by NaCI.关键词
壳聚糖/牛血清蛋白/吸附/正交实验/离子强度Key words
chitosan/bovine serum albumin/adsorption/orthogonal experiment/ionic strength分类
化学化工引用本文复制引用
刘麒,禹雪晴,荆迎军,夏文晨,杨景涛..交联壳聚糖对牛血清蛋白的吸附及释放行为[J].食品工业科技,2012,33(23):117-121,5.基金项目
河北省引进留学人员经费资助项目(冀人社字[2011]226号). ()