食品工业科技2012，Vol.33Issue(23)：158-162,5.
利用工程大肠杆菌生产不同类型的游离脂肪酸
Production of different types of free fatty acids by engineered E.cofi
摘要
Abstract
Free fatty acids(FFAs) play a key role in the flavor of many foods.To better meet the needs of flavor industry,it is needed to produce more different types of FFAs.Seven engineered E.coli strains were successfully constructed by overexpressing seven different thioesterases, respectively.Next,taking one engineered strain as an example,an optimization of the production conditions was carried out to improve the production of FFAs.The optimized conditions were 15g/L Na2HPO4 and 7.5g/L KH2PO4 as the concentration of the phosphates; the engineered strains were induced with 0.25mmol/L IPTG at 30~C.Under this condition,the FFAs production of seven engineered strains was determined by gas chromatography( GC).The strains LL1, LL2 and LL4, which overexpress BTE, AtFatA and ' tesA, respectively, obviously enhanced the production of FFAs, and LL1 achieved 346.4mg/L FFAs, representing the highest FFA titer obtained in all engineered strains.In addition, BTE,AtFatA and 'TesA were found to have different substrate specificities by analyzing the composition of FFAs they achieved,and a range of C12-C18 FFAs was obtained in these engineered strains.Therefore, more types of FFAs,which didn't exist in animal and plant oils,were finally obtained by metabolic engineering of E.coll关键词
游离脂肪酸/工程大肠杆菌/硫酯酶Key words
free fatty acids/engineered E. coli/thioesterase分类
轻工纺织引用本文复制引用
李玲玲,刘强,郑艳宁,秦文,咸漠..利用工程大肠杆菌生产不同类型的游离脂肪酸[J].食品工业科技,2012,33(23):158-162,5.基金项目
国家自然科学基金 （）
广东省中国科学院全面战略合作项目（20098091300146）. （）
