食品科学2012，Vol.33Issue(23)：125-128,4.
双向电泳分析宣威火腿蛋白质组方法的建立
Proteomic Analysis of Xuanwei Ham by Two-Dimensional Gel Electrophoresis
摘要
Abstract
In the present study,a method for the proteomic analysis of Xuanwei ham was established using two-dimensional gel electrophoresis(2-DE).Muscle protein from Xuanwei ham was extracted with trichloroacetic acid-acetone,quantified,and analyzed by isoelectric focusing electrophoresis and SDS-PAGE.After electrophoresis,the gels were stained with coomassie brilliant blue,and the observed protein spots were analyzed using imageMaster 2D platinum software and one of them was selected and analyzed by matrix-assisted laser desorption ionization-time of flight-time of flight-mass spectrometry(MALDI-TOF/TOF MS) after tripsin digestion.The obtained peptide mapping fingerprint was analyzed using Mascot software.A total of 1749 clear protein spots were observed in the 2-DE pattern of Xuanwei ham,which were mainly distributed over a pH range of 4.0-7.0.The tested protein spot was identified as gamma-2-actin protein and found to have a Mascot score of 129(threshold was 67).These results demonstrate the successful establishment of a 2-DE method for the proteomic analysis of Xuanwei ham.关键词
宣威火腿/蛋白质组学/双向电泳Key words
Xuanwei ham/proteomics/two-dimensional electrophoresis分类
轻工纺织引用本文复制引用
廖国周,王桂瑛,程志斌,施忠芬,曹锦轩..双向电泳分析宣威火腿蛋白质组方法的建立[J].食品科学,2012,33(23):125-128,4.基金项目
云南省社会发展科技计划应用基础研究专项 （）
云南省现代农业（生猪）产业技术体系建设专项 （）
