食品科学2012，Vol.33Issue(23)：166-170,5.
响应面法优化微量热仪中葡萄糖氧化酶催化反应的条件
Optimization of Catalytic Reaction Conditions for Glucose Oxidase in Microcalorimeter by Response Surface Methodology
摘要
Abstract
Response surface methodology based on a central composite design was used to optimize catalytic reaction conditions for glucose oxidase in micro-differential scanning calorimeter(Micro DSC) based on heat release.Three reaction conditions in decreasing order of their effects on heat release were enzyme dosage,pH and reaction temperature and their optimal values were 1.58 mg/mL,4.46 and 35 ℃,respectively.Under these conditions,the observed total heat release was 39.6668 J,and the relative error was 1.11% compared with 40.1071 J of the predicted value.The fitted regression model was statistically significant.v关键词
微量热法/葡萄糖氧化酶/响应面Key words
microcalorimetric method/glucose oxidase/response surface methodology分类
轻工纺织引用本文复制引用
张成楠,徐斐,曹慧,于劲松..响应面法优化微量热仪中葡萄糖氧化酶催化反应的条件[J].食品科学,2012,33(23):166-170,5.基金项目
国家自然科学基金项目 （）
