食品科学2012，Vol.33Issue(23)：175-179,5.
三酶法制备罗非鱼鱼皮胶原蛋白抗氧化肽及活性研究
Three-Step Enzymatic Hydrolysis of Tilapia Skin Collagen for Preparation of Antioxidant Peptides and Their Antioxidant Activity
摘要
Abstract
This study explored optimal conditions for the enzymatic preparation of antioxidant peptides from tilapia skin collagen and the antioxidant activity of antioxidant peptides.Sequential hydrolysis with alkaline protease,trypsin and Orientase 20A was the best way to prepare antioxidant peptides from tilapia skin collagen,as shown by a comparison of the effects of single use and sequential combinations of proteases on DPPH radical scavenging activity of hydrolyzed tilapia skin collagen.In the final step(Orientase 20A) of the hydrolysis process,process parameters including temperature,time,pH and enzyme/substrate ratio were optimized using response surface methodology based on degree of hydrolysis(DH) and DPPH radical scavenging activity of hydrolyzed tilapia skin collagen to be 41.74 ℃,3.97,6 h and 1.5%,respectively.Under these hydrolysis conditions,the predicted DH and DPPH radical scavenging activity of hydrolyzed tilapia skin collagen were 9.42% and 35.01%,respectively,and the observed values were 9.57% and 35.21%,respectively.Thus,the developed response surface model is feasible and can be used for practical prediction.The tilapia skin collagen hydrolysate obtained under the optimized hydrolysis conditions had potent scavenging activity against DPPH and ABTS+radicals with an IC50of 10.78 mg/mL and 8.26 mg/mL,respectively.关键词
三酶法/响应面法/水解物/抗氧化活性/罗非鱼皮胶原蛋白/肽Key words
three-step enzymatic hydrolysis response/surface methodology/hydrolysate/antioxidant activity/tilapia skin collagen/peptide分类
轻工纺织引用本文复制引用
夏光华,申铉日,蔡锦红,徐克寒,张诚..三酶法制备罗非鱼鱼皮胶原蛋白抗氧化肽及活性研究[J].食品科学,2012,33(23):175-179,5.基金项目
“十二五”国家科技支撑计划项目 （）
海口市重点科技计划项目 （）
