食品科学2012，Vol.33Issue(23)：209-214,6.
响应面法优化猪血红蛋白制备ACE抑制肽的酶解工艺条件
Optimization of Enzymatic Hydrolysis Conditions for ACE Inhibitory Peptides Preparation from Porcine Hemoglobin by Response Surface Methodology
摘要
Abstract
The enzymatic preparation of angiotensin I-converting enzyme(ACE) inhibitory peptides from porcine hemoglobin using pepsin was optimized by response surface methodology.ACE inhibitory rate in vitro and degree of hydrolysis(DH) were investigated with respect to hydrolysis parameters including temperature,pH,substrate concentration,enzyme dosage and time.As a result,a quadratic polynomial model was developed.The optimum conditions for enzymatic preparation of ACE inhibitory peptides from porcine hemoglobin were found to be hydrolysis at pH 1.98 and 37.60 ℃ for 4 h with a substrate concentration of 4.98 g/100 mL and a enzyme dosage of 3.04%.Under these conditions,the prepared peptides showed an ACE inhibitory rate of 70.09%.关键词
猪血红蛋白/ACE抑制肽/酶解工艺/响应面法Key words
porcine hemoglobin/ACE inhibitory peptide/enzymatic hydrolysis/response surface methodology分类
轻工纺织引用本文复制引用
郑炯,邓惠玲,阚建全..响应面法优化猪血红蛋白制备ACE抑制肽的酶解工艺条件[J].食品科学,2012,33(23):209-214,6.基金项目
西南大学基本科研业务费专项 （）
