食品科学2012，Vol.33Issue(23)：266-269,4.
胰蛋白酶在磁性核壳介孔分子筛上的固定化研究
Immobilization of Trypsin onto Magnetic Core-shell Mesoporous Molecular Sieve
摘要
Abstract
A dipping method was used to immobilize trypsin onto magnetic core-shell mesoporous molecular sieve(Fe3O4@ MCM-41).The effects of immobilization conditions on the recovery of trypsin activity were investigated and immobilized trypsin was analyzed for enzymatic properties and carrier structure.The recovery of trypsin activity reached 55.9% when immobilizing at pH 8.2 and an enzyme loading of 60 mg/g for 4 h.Energy dispersive X-ray spectroscopy(EDS) analysis showed that the immobilized trypsin contained 14% trypsin(calculated on the basis of total nitrogen content).Compared with free trypsin,it showed tolerance to temperature and pH in much wider ranges and better repeated usability and meanwhile,the mesopore structure of the carrier remained good.关键词
胰蛋白酶/磁性核壳/介孔分子筛/固定化Key words
trypsin/magnetic core-shell/mesopore molecular sieve/immobilization分类
化学化工引用本文复制引用
安红,宋伟明,高树刚..胰蛋白酶在磁性核壳介孔分子筛上的固定化研究[J].食品科学,2012,33(23):266-269,4.基金项目
黑龙江省自然科学基金项目 （）
黑龙江省教育厅面上项目 （）
