食品科学2012，Vol.33Issue(24)：66-71,6.
响应面法同步优化裙带菜中蛋白和多糖提取工艺
Optimization of Simultaneous Extraction of Proteins and Polysaccharides fromUndaria pinnatiifda by Response Surface Methodology
摘要
Abstract
Response surface methodology was used to optimize the simultaneous alkaline extraction of proteins and polysaccharides fromUndaria pinnatifidabased on extraction efficiency. All four operating parameters including alkaline concentration, extraction time, extraction temperature and solvent-to-solid ratio on had a significant effect on the extraction yields of proteins and polysaccharides. The optimum process conditions for simultaneously increasing the yields of proteins and polysaccharides were found to be extraction at 55 ℃ for 5 h with an alkaline concentration of 2.2% and a solvent-to-solid ratio of 23:1 (mL/g). The actual yields of proteins and polysaccharides under the optimized conditions were 63.21% and 29.11%, respectively. Good agreement with the predicted values was found. Therefore, response surface methodology is a feasible way to optimize the simultaneous alkaline extraction of proteins and polysaccharides fromUndaria pinnatifida.关键词
裙带菜/蛋白/多糖/提取/响应面法/叠加等高线图Key words
Undaria pinnatifida/protein/polysaccharides/extraction/response surface methodology/overlay contour plot分类
轻工纺织引用本文复制引用
王明艳,周琴,杨俊,蔡晓庆,谭琪,张秋荣..响应面法同步优化裙带菜中蛋白和多糖提取工艺[J].食品科学,2012,33(24):66-71,6.基金项目
江苏省海洋资源开发研究院科技开放基金项目(JSIMR10E01)；江苏省海洋生物技术重点建设实验室开放课题(2010HS11)；淮海工学院大学生创新项目；连云港市科技攻关项目农业攻关课题(CG1133) （JSIMR10E01）
