食品科学2012，Vol.33Issue(24)：82-85,4.
鱼油中二十碳五烯酸乙酯和二十二碳六烯酸乙酯的超临界流体色谱分离技术
Supercritical Fluid Chromatographic Separation of Eicosapentaenoic Acid Ethyl Ester and Docosahexaenoic Acid Ethyl Ester from Fish Oil
摘要
Abstract
A supercritical fluid chromatographic method to separate eicosapentaenoic acid ethyl ester (EPA-EE) and docosahexaenoic acid ethyl ester (DHA-EE) from fish oil was established. Hedera ODS-BP was found to be the most effective column for separating EPA-EE and DHA-EE from fish oil as demonstrated by a comparison with Luna 5u Silica, Hypersil ODS2, ZORBAX SB-C8, and Hypersil C8. The effects of mobile phase modifier type, methanol percentage in mobile phase, back pressure and column temperature for the separation of both esters by Hedera ODS-BP on capacity factor, diastereoselectivity and resolution were investigated. The optimum conditions forthese factors were found to be separation on Hedera ODS-BP column at a column temperature of 40 ℃ an a back pressure of 100 bar using a mobile phase containing 10% methanol as a modifier at a total flow rate of 2 mL/min. Under these conditions, baseline separation of EPA-EE and DHA-EE was achieved. The developed method was simple, rapid, highly reproducible and suitable for the separation of EPA-EE and DHA-EE from fish oil.关键词
超临界流体色谱(SFC)/二十碳五烯酸乙酯(EPA-EE)/二十二碳六烯酸乙酯(DHA-EE)/分离Key words
supercritical fluid chromatography (SFC)/eicosapentaenoic acid ethyl ester (EPA-EE)/docosahexaenoic acid ethyl ester (DHA-EE)/separation分类
化学化工引用本文复制引用
陈伟珠,洪专,赵敏,张怡评,谢全灵,易瑞灶..鱼油中二十碳五烯酸乙酯和二十二碳六烯酸乙酯的超临界流体色谱分离技术[J].食品科学,2012,33(24):82-85,4.基金项目
福建省科技计划项目(2009Y0035)；海洋公益性行业科研专项(200905022) （2009Y0035）
