食品科学2012，Vol.33Issue(24)：107-110,4.
梅花鹿胎盘免疫调节因子制备工艺
Preparation of Immunoregulatory Factors from Sika Deer Placenta
摘要
Abstract
Immunoregulatory factors as bioactive smal molecules were prepared from sika deer placenta homogenate using ultrasonic-assisted acetone extraction. Process conditions such as ultrasonic power, homogenate-to-normal saline ratio, utrasonic treatment time and homogenate-to-acetone ratio were optimized using an orthogonal array design to be 400 W, 1:1 (g/mL), 120 min and 1:3 (V/V), respectively. The polypeptide concentration of the extract obtained under these conditions was 30.35 mg/mL. The extract was further ultrafiltrated and a clear yellowish liquid containing immunoregulatory factors was obtained. The liquid contained mainly (3.7 ± 0.04) mg/mL peptides, (3.3 ± 0.07)μg/mL nucleic acid and (0.54 ± 0.002) mg/mL polysaccharides, whereas smaler amounts of polypeptides, nucleic acid and polysaccharides were found in the retentate of the extract after dialysis.关键词
梅花鹿胎盘/免疫调节因子/正交试验/制备工艺Key words
sika deer placenta/immunoregulatory factors/orthogonal array design/preparation process分类
轻工纺织引用本文复制引用
孙辞,边媛媛,吕永通,胡风庆..梅花鹿胎盘免疫调节因子制备工艺[J].食品科学,2012,33(24):107-110,4.基金项目
沈阳市发展与改革委员会高技术研发基金项目(2010-16)；辽宁省教育厅基金项目(L2010150) （2010-16）
