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生鲜猪肉中沙门氏菌的分离、鉴定及耐药性检测

姚大伟 江芸 徐幸莲 周光宏

食品科学2012,Vol.33Issue(24):210-214,5.
食品科学2012,Vol.33Issue(24):210-214,5.

生鲜猪肉中沙门氏菌的分离、鉴定及耐药性检测

Isolation, Identification and Antibiotic Resistance of Salmonella from Fresh Pork

姚大伟 1江芸 2徐幸莲 1周光宏1

作者信息

  • 1. 南京农业大学 肉品加工与质量控制教育部重点实验室,江苏 南京 210095
  • 2. 南京师范大学金陵女子学院,江苏 南京 210097
  • 折叠

摘要

Abstract

The aim of this study was to investigate Salmonella contamination in fresh pork in Nanjing and test the antibiotic resistance of Salmonella isolates. RT-PCR was used to primarily examine whether fresh pork samples collected from local supermarkets or farmers’ markets were contaminated by Salmonella. Salmonella was isolated and identified from contaminated samples according to relevant national standards and tested for antibiotic resistance. A total of 21 of 83 pork samples were found to be positive for Salmonella with a positive rate of 25.3%. Seven of the positive samples were resistant to at least of one of 11 tested antibiotics with a resistance rate of 33.3%. Our results suggest the serious microbial contamination in fresh pork in Nanjing with high positive rate and tetracycline resistance. In addition, chilled pork was superior to fresh pork in terms of aerobic plate count and positive rate. No significant differences were found between fresh pork marketed in local supermarkets and farmers’ markets.

关键词

猪肉/沙门氏菌/耐药性

Key words

pork/Salmonella/antibiotic resistance

分类

轻工纺织

引用本文复制引用

姚大伟,江芸,徐幸莲,周光宏..生鲜猪肉中沙门氏菌的分离、鉴定及耐药性检测[J].食品科学,2012,33(24):210-214,5.

基金项目

国家公益性行业(农业)科技专项(200903012);国家自然科学基金面上项目(31071614) (农业)

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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