食品科学2012，Vol.33Issue(24)：283-285,3.
核固红光度法测定乳制品中微量铬
Spectrophotometric Determination of Trace Chromium in Dairy Products Using Nuclear Fast Red
摘要
Abstract
A new spectrophotometric method for the simultaneous determination of chromium (Ⅲ) and chromium (Ⅵ) in dairy products was proposed. This method was based on the oxidative decoloration of nuclear fast red by chromium (Ⅵ) in hydrochloric acid solution, which is linearly related to chromium (Ⅵ) concentration in a certain range. The oxidization of chromium (Ⅲ) to chromium (Ⅵ) by hydrogen peroxide in sodium hydroxide solution allows the determination of total chromium and the consequent calculation of chromium (Ⅲ). The wavelength of maximum absorbance was 560 nm with an apparent molar absorption coefifcient of 2.2 × 104 L/(mol•cm). The absorbance obeyed Beer-Lambert law over the range of 0.2–1.6 µg/mL for chromium (Ⅵ). The proposed method was applied to determine chromium (Ⅲ) and chromium (Ⅵ) in several kinds of dairy products and the results were in good agreement with those determined by 1,5-diphenylcarbazide spectrophotometry.关键词
核固红/铬(Ⅲ)/铬(Ⅵ)/分光光度法/乳制品Key words
nuclear fast red/chromium (Ⅲ)/chromium (Ⅵ)/spectrophotometry/dairy products分类
化学化工引用本文复制引用
刘英红,马卫兴,斯琴高娃..核固红光度法测定乳制品中微量铬[J].食品科学,2012,33(24):283-285,3.基金项目
淮海工学院自然科学研究项目(2010150014；2010150018)；江苏省海洋资源开发研究院科技开放基金项目(SIMR10E07) （2010150014；2010150018）
