食品科学2012，Vol.33Issue(24)：352-355,4.
硅胶载体氯霉素免疫亲和柱的制备
Preparation of Silica Gel Immunoaffinity Column for Chloramphenicol Determination
摘要
Abstract
Silica gel was used as a carrier for preparing an immunoaffinity column (IAC) to determine chloramphenicol. Amino groups were introduced to the surface of silica gel using 3-aminopropyltriethoxysilane (APTES) as a silane coupling agent and the modified silica gel was conjugated with anti-chloramphenicol antibodies using glutaraldehyde. As a result, a conjugation ratio of 72% was obtained. The maximum adsorption capacity of the prepared IAC was 80 ng/mL. The elution solvent was PBS containing 70% methanol. After sample pretreatment by three different methods, chloramphenicol residues in milk were purified and enriched using the IAC and determined by ELISA.关键词
免疫亲和柱/硅胶/氯霉素抗体/戊二醛法Key words
immunoaffinity column/silica gel/anti-chloramphenicol antibody/glutaraldehyde分类
轻工纺织引用本文复制引用
张灿,周婷,陆介宇,段玉清,王硕,于娇..硅胶载体氯霉素免疫亲和柱的制备[J].食品科学,2012,33(24):352-355,4.基金项目
国家自然科学基金青年科学基金项目(31000783)；江苏大学优秀人才基金项目(09JDG052)；江苏大学第11批大学生科研立项项目(11A349) （31000783）
