摘要
Abstract
In this paper, Candida rugosa lipase (CRL) catalyzed reaction of vitamin E and ferulic acid ethyl ester were studied to synthesize alpa-tocopheryl ferulate in the reverse micelle system. Through single factor test and the use of the enzyme in AOT-iso-octane-water reverse micelle system, vitamin E and ferulic acid ethyl ester were catalyzed to synthesize alpa-tocopheryl ferulate after the 136 h reaction. And the influential factors of the synthetic product, such as the moisture content, speed, temperature, buffer pH, AOT concentration reaction conditions, were studied. Through the uniform design experiment and quadratic polynomial stepwise regression analysis of the results, the optimal reaction conditions of synthetic product were gained as follows: moisture content 10%, AOT concentration of 80mmol/L, the buffer pH5.0, temperature 25℃, speed 270 rpm, under which the VE Ferulate obtained was 15.36%.关键词
丁二酸二(2-乙基己基)酯磺酸钠/VE阿魏酸酯/脂肪酶Key words
AOT/ alpa-tocopheryl ferulate/ lipase
