食品工业科技2013，Vol.34Issue(2)：68-71,74,5.
纤维素酶的CMC酶活测定条件的研究
Study on the optimum conditions of determination of cellulase activity
孙盈 1田永强 1赵丽坤1
作者信息
- 1. 兰州交通大学化学与生物工程学院,甘肃兰州730070
- 折叠
摘要
Abstract
The experimental conditions of CMC enzyme activity assay were researched. Six influencing factors: enzymatic reaction time,reaction-temperature,pH.detective wavelength,substrate concentration,crude enzyme and substrate addition were studied by a single factor test, Finally,orthogonal experiment,variance analysis and multiple comparisons were combined together,and the optimum conditions of determination of CMC enzyme activity were determined. The result showed that,when the volume of crude enzyme and substrate were 2mL and pH6.2,the primary and secondary factors which affected cellulase activity were enzymatic reaction time, reaction temperature, enzymatic determination of wavelength and substrate concentration. Experiments showed that;the enzymatic reaction temperature 40℃,pH6.2,substrate concentration 10g/L,the volume of crude enzyme and substrate were 2mL,enzymatic reaction time 30min,and the detective wavelength at 520nm were optimum conditions for the determination.关键词
纤维素酶/酶活力测定/条件Key words
cellulase/determination of enzyme activity/condition分类
轻工纺织引用本文复制引用
孙盈,田永强,赵丽坤..纤维素酶的CMC酶活测定条件的研究[J].食品工业科技,2013,34(2):68-71,74,5.
