食品工业科技2013，Vol.34Issue(2)：171-174,178,5.
产右旋糖酐酶埃氏交替单胞菌的筛选、鉴定及酶活分析
Screening, identification and activity analysis of a Dextranase-producing Altermonas espejiana strain
摘要
Abstract
A dextranase producing strain Altermonas espejiana YSN412 was isolated from a beach mud sample of Lianyungang port. The enzymatic properties of the dextranase AesDEX were analyzed. The culture condition for production of enzyme from the strain was investigated. The optimum temperature and pH value of the enzyme were 30℃ and 8 respectively. After incubation at 30℃ and 50℃ for 2.5h,the remnant relative enzymatic activities were 95% and 14% respectively. The optimum carbon and nitrogen sources were cellobiose and yeast powder respectively. When the concentration of NaCI was 2%(w/w) and the initial pH value was 8,the enzymatic activity of the liquid culture reached its maximum 9U/mL after culturmg the strain for 12h. The A espejiana YSN412 strain isolated and identified in this study produced dextranase quickly and the dextranase AesDEX had a low optimum temperature for enzymatic activity. The enzyme showed the potential to lower the cost and increase the efficiency in industry.关键词
埃氏交替单胞菌/右旋糖酐酶/酶学性质/产酶培养Key words
Altermonas espejiana/dextranase/enzymatic property/enzyme producing cultivation分类
轻工纺织引用本文复制引用
焦豫良,王淑军,吕明生,房耀维,刘姝..产右旋糖酐酶埃氏交替单胞菌的筛选、鉴定及酶活分析[J].食品工业科技,2013,34(2):171-174,178,5.基金项目
国家863计划项目(2011AA09070302) （2011AA09070302）
连云港市工业攻关项目(CG1003). （CG1003）
