沈阳农业大学学报2012，Vol.43Issue(5)：534-540,7.
真菌广谱拮抗菌GQ-17的鉴定、发酵液性质研究及发酵条件优化
Identification, Physical and Chemical Properties and Fermentation Condition Optimization of Antagonistic Strain GQ-17
摘要
Abstract
The aim of this study was to evaluate the potential of an antagonism strain GQ-17 for biocontrol ability against plant fungus disease.Taxonomic identification based on morphology, physiological and biochemical characterization, cell components analysis together with 16S rDNA sequence analysis were conducted.The physical and chemical properties of the crude fermentation extract were measured with oxford cup method.In addition, the single factor test and orthogonal design experiments were also employed to screen out the optimal liquid fermentation compositions and the optimal fermentation conditions of the strain.The result showed that strain GQ-17 was identified as Streptomyces rimosus.Its crude fermentation extract had high inhibitory activities on Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Fusarium graminearum, Curvularia lunata, Verticillium dahliae, Corynespora cassiicola and Fusarium oxysporum f.sp.cucumerinum.It was thermostable with 88.6% remaining activities after heat threated at 100℃ for 30min.It was insensitive to pH and stable in the pH range of 5-9.Its activities reduced 51.5% under sunlight irradiation 6h.The best compositions of cultural medium were as follow: com meal 10g,soybean flour 10g, NaCl 4g,CaCO3 1g,deionized water 1000mL.The optimal fermentation conditions were as follow: initial pH7.0, temperature 31℃, fermentation time 5d, inoculation rate 3%, medium capacity 50mL/250mL and rotation speed 150r·min-1.关键词
生物防治/放线菌/分类鉴定/发酵条件优化/龟裂链霉菌Key words
biological control/ actinomycetes/ identification/ fermentation condition optimization/ Streptomyces rimosus分类
农业科技引用本文复制引用
闫建芳,刘秋,赵柏霞,刘志恒,齐小辉,刘长建,周济廷..真菌广谱拮抗菌GQ-17的鉴定、发酵液性质研究及发酵条件优化[J].沈阳农业大学学报,2012,43(5):534-540,7.基金项目
国家自然科学基金项目(31070005,21276047,31270057) （31070005,21276047,31270057）
中央高校基本科研业务费专项项目(DC12010103) （DC12010103）
