食品工业科技2013，Vol.34Issue(3)：150-154,5.
重组原核表达载体pQE-30-luxS的构建与表达
Construction and expression of recombinant prokaryotic vector pQE-30-luxS
摘要
Abstract
To construct the prokaryotic expression vector pQE-30-luxS and express the fusion protein.The luxS gene was amplified by polymerase chain reaction(PCR) using the DNA of Lactobacillus plantarum KLDS1.0391 as template,then it was cloned to vector pMD18-T simple.After the luxS gene was identified to be correct,the target gene was connected with expression vector pQE-30 and the recombinant plasmid pQE-30- luxS was transformed into E.coli M15.The recombinant expressed proteins induced by Isopropyl β-D-1-Thiogalactopyranoside( IPTG) were analyzed and identified with SDS-PAGE.The results showed that the prokaryotic expression vector pQE-30-luxS was successfully constructed.The sequence of cloned luxS was identical with the known sequences, and LuxS protein was successfully expressed in E.coli M15.The results referred to above underlaid the relationship between the synthesis of Al-2 in vitro and the synthesis of bacteriocins of Lactobacillus plantarum KLDS1.0391.关键词
luxS基因/重组质粒/基因表达Key words
luxS gene/recombinant plasmid/gene expression分类
轻工纺织引用本文复制引用
赵日红,孟祥晨,满丽莉..重组原核表达载体pQE-30-luxS的构建与表达[J].食品工业科技,2013,34(3):150-154,5.基金项目
黑龙江省教育厅科学技术研究项目(1252lz002) （1252lz002）
哈尔滨市科技创新人才研究专项资金项目(RC2011LX020002) （RC2011LX020002）
