食品工业科技2013，Vol.34Issue(3)：306-309,4.
RP-HPLC测定保加利亚乳杆菌腺苷酸方法的建立与应用
Determination of adenosine phosphates in L.bulgaricus by RP-HPLC method and its application
摘要
Abstract
To evaluate the quenching effect of the quencheing solutions in the metabolomics research,a RP-HPLC method was developed for the determination of adenosine phosphates in Lbulgaricus.Tbe chromategrap-hic separation was achieved on an SinoChrom ODS-BP C18 column and a mobile phase of methanol-30mmol /L phosphate( V/V = 5:95) buffer solution ( pH7) with a flow rate of 0.7mL/min. The column temperature and UV detection wavelength were set as 25℃ and 254nm,respectively.The method had the good linearity and higher accuracy.The recovery rates of adenosine phosphates were in the range of 94.12% ~ 109.6% .The intracellular metabolite leakages of Lbulgaricus was evaluated by the RP-HPLC method,when 60% (V/V) methanoi/water at -40℃ that was the most popular method to quench the metabolism.The results showed that the leak rate of ATP was as high as 70.43% ±2.3% ,EC value was 0.42 ±0.015.11 was inferred that the quenching fluid could not only cause severe leakage of intracellular metabolites but also not completetly quench the metabolism .The quenching procotol was not suitable for quenches Lbulgaricus.关键词
保加利亚乳杆菌/反相高效液相色谱/腺苷酸/淬灭Key words
L.bulgaricus/ reversed phase high performance liquid chromatography/ adenosine phosphates/ quenching分类
轻工纺织引用本文复制引用
孙茂成,李艾黎,霍贵成,孟祥晨,戚晓熙..RP-HPLC测定保加利亚乳杆菌腺苷酸方法的建立与应用[J].食品工业科技,2013,34(3):306-309,4.基金项目
教育部创新团队科学基金资助(IRT-0959-205) （IRT-0959-205）
国家自然科学青年基金(31101267). （31101267）
