食品工业科技2013，Vol.34Issue(3)：314-316,3.
高效液相色谱-蒸发光散射测定瓜蒌中L-瓜氨酸的含量
Separation and identification of L-citrulline in Trichosanthis Fructus by high performance liquid chromatography-evaporative light scattering detection
摘要
Abstract
A high performance liquid chromatography-evaporative light scattering detection method for separation and determination of L-citmiiine in Trichosanthis Fructus was established. The analytical column was Agilent ZORBAX SB-Aq(250mm ×4.6mm i.d.,5μm).The mobile phase was water,and the flow rate was 1.0mL/min.The sample was determined by an evaporative light scattering detector.The temperature of drift tube was kept at 115℃ and the flow rate of atmosphere was 3.2L/min.The result showed that under the optimized conditions,the logarithm correlation between peak area and the concentration of L-citrulline was in a good linearity.The calibration curve had good linearity in the range of 0.05 ~0.75mg/mL, the detection limit of L-citrulline was 12.9μg/mL, and the average recovery was 98.36% ( n =5, RSD = 1.77%) .The method was applied in the detection of L-citrulline in Trichosanthis Fructus,and the result revealed that the content was about 0.1mg/g.The method is a convenient, rapid and effective method for qualitatively analysis of L-citrulline in Trichosanthis Fructus.关键词
L-瓜氨酸/瓜蒌/高效液相色谱/蒸发光散射检测Key words
L-citrulline/Trichosanthis Fructusjhigh performance liquid chromatography/evaporative light scattering detection分类
轻工纺织引用本文复制引用
李爱峰,柳仁民,张永清..高效液相色谱-蒸发光散射测定瓜蒌中L-瓜氨酸的含量[J].食品工业科技,2013,34(3):314-316,3.基金项目
"华中区域中药材规范化种植及大宗中药材综合开发利用技术研究山东省科技发展计划项目"课题(2011BAI06B00) （2011BAI06B00）
"山东道地药材资源质量控制科技平台建设"课题(2008GG2NS02022). （2008GG2NS02022）
