食品工业科技Issue(4)：66-68,75,4.
高效液相色谱法同时测定绿原酸及其五种主要代谢产物
Simultaneous analysis of chlorogenic acid and its five major metabolites compounds by high performance liquid chromatography
罗晓燕 1吴九九 1温艳梅 1阮征 1骆成尧 1周艳 1蔡力创 1刘文群 1印遇龙1
作者信息
摘要
Abstract
A method had been developed for the simultaneous determination of the contents of chlorogenic acid,caffeic acid,3-hydroxyphenylpropionic acid,p-coumaric acid,ferulic acid and hippuric acid by high performance liquid chromatography. Six phenolic compounds were separated on a Agilent Eclipse XDB-C18 (250×4.6mm,5μm) column using CH3OH and 1% acetic acid aqueous solution as mobile phase with near gradient elution,and detected by ultraviolet detector(UV) at wavelength of 320nm and 253nm. There were good linear correlations between the concentrations and peak areas of the six phenolic compounds with the correlation coefficients of 0.9979～0.9999. The average recoveries were 86.84%～101.56% with relative standard deviations of 1.15%～2.29%. The results suggested that this method was characteristic of rapidity,accuracy, high sensitivity. The method can be used for qualitative detection of chlorogenic acid and its metabolites.关键词
绿原酸/代谢物/高效液相色谱/测定Key words
chlorogenic acid/metabolites/high performance liquid chromatography/determination分类
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罗晓燕,吴九九,温艳梅,阮征,骆成尧,周艳,蔡力创,刘文群,印遇龙..高效液相色谱法同时测定绿原酸及其五种主要代谢产物[J].食品工业科技,2013,(4):66-68,75,4.
