食品工业科技Issue(4)：243-248,6.
杜仲叶总黄酮微波辅助提取工艺的优化及其抗氧化活性研究
Optimization of microwave-assisted extraction of flavonoids from Eucommia ulmoides leaf and investigation of their antioxidative effect
摘要
Abstract
Using response surface methodology (RSM) to deal with the optimization of process conditions for the microwave-assisted extraction of total flavonoids from Eucommia ulmoides leaf,and their antioxidative effect in vitro was investigated. Based on single factor experiments,a quadratic regression model was established to indicate the individual and interactive effects of solid to liquid ratio,extraction time,microwave power,and ethanol concentration on the yields of naringin and hesperidin according to the Box-Behnken experimental design. From the response surface for the model,the optimal extraction conditions were obtained as follows: powder size 100 mesh,solid to liquid ratio 60:1 (mL/g) ,extraction time 5.3min,microwave power 757W,and ethanol concentration 67%(v/v). Under these conditions,the actual yields of naringin and hesperidin were 69.02mg/g and 4.96mg/g respectively,which were agreement with the theoretically predicted values. Eucommia ulmoides leaf flavonoids revealed a relatively better scavenging effect on DPPH radicals and hydroxyl free radicals than VC. Therefore, Eucommia ulmoides leaf flavonoids are effective as novel natural antioxidants.关键词
响应面法/微波辅助提取/杜仲叶/总黄酮/抗氧化Key words
response surface methodology / microwave - assisted extraction / Eucommia ulmoides leaf/ total flavonoids/antioxidative effcet分类
轻工纺织引用本文复制引用
李旭,刘停..杜仲叶总黄酮微波辅助提取工艺的优化及其抗氧化活性研究[J].食品工业科技,2013,(4):243-248,6.基金项目
国家自然科学基金(21101090) （21101090）
江西省自然科学基金项目(2009GQY0190). （2009GQY0190）
