食品工业科技2013，Vol.34Issue(6)：65-69,5.
表面增强拉曼光谱快速鉴别无乳链球菌的研究
Rapid identification of Streptococcus genus based on surface-enhanced Raman spectroscopy
摘要
Abstract
Rapid identification of foodborne pathogens Streptococcus spp. by surface - enhanced Raman spectroscopies (SERS) was studied. Gold sol was prepared by tri - sodium citrate, and mixed with Streptococcus spp. the SERS signals were collected. First, methods of activation,pre-culturing combinations and the preparation of the colony of Streptococcus agalactia were determined,then the identification of Streptococcus genus from other common pathogenic bacteria of Gram-positive was done by cluster analysis (HCA) and principal component analysis (PCA), and also the identification could realize between different streptococcal pathogens. The characteristic peaks could be identified at 6h after artificial inoculation Streptococcus agalactiae into commercial full-fat pasteurized milk, which improved effectively the speed of detection. Our results provided the basic information of rapid identification Streptococcus in food,which showed that the SERS technology could be useful in monitoring the foodborne pathogens and in early warning of food safety.关键词
表面增强拉曼光谱/金胶粒子/链球菌属/主成分分析/系统聚类分析/无乳链球菌Key words
surface enhanced Raman spectroscopy/ gold colloid particles/ Streptococcus-, principal component analysis/ hierarchical cluster analysis/ Streptococcus agalactiae.分类
化学化工引用本文复制引用
王毅谦,徐丽,谢云飞,邵景东,姚卫蓉..表面增强拉曼光谱快速鉴别无乳链球菌的研究[J].食品工业科技,2013,34(6):65-69,5.基金项目
江苏出入境检验检疫局科技计划项目(2010KJ39). （2010KJ39）
