食品工业科技2013，Vol.34Issue(6)：70-72,76,4.
微生物酶制剂中转基因微生物的实时荧光PCR检测方法
A method of real-time PCR for detection of genetically modified microorganisms in microbiology derived enzyme
摘要
Abstract
A new method of real-time PCR was built to detect genetically modified microoganisms in microbioloogy
derived enzyme. The primer and the MGB probe were desgined on the sequence of the alcohol oxidase-1
(AOX1) promoter. The experimental result showed that the sensitivity of detction was 1pg Pichia pastoris DNA,
and could accurately analyze the residue status of genetically modified microorganism in microbiology derived
enzyme from different separation stage. This practical method could be used to monitor separation of
transgenic mocrobiology in microbiology dereirved enzyme, and provide reference for the detction of genetically
modified microorganism related stdudies.
关键词
微生物酶制剂/转基因微生物/实时荧光PCR/醇氧化酶-1启动子/MGB探针Key words
microbiology derived enzyme/genetically modified microognsims/ real-time polymerase chain reaction
(RT-PCR)/ alcohol oxidase-1 (AOX1) promoter/ MGB Probe分类
轻工纺织引用本文复制引用
张清平,张奕南,曲勤凤..微生物酶制剂中转基因微生物的实时荧光PCR检测方法[J].食品工业科技,2013,34(6):70-72,76,4.
