食品工业科技2013，Vol.34Issue(6)：85-87,107,4.
白叶单枞黑茶降血糖活性成分的高速逆流色谱分离
Separation of bioactive compounds from dark tea prepared from Camellia sinensis var.baiye dancong using HSCCC target-guided by α-glycosidase inhibitory activity
摘要
Abstract
α-glycosidase inhibitors in dark tea were studied systematically by the method of a combination of extraction with 60% ethanol.fractionation on D101 column,and further purification by HSCCC ordinally. The result showed that fractions D-6.D-7 and D-8 obtained by D101 column chromatography indicated higher inhibitory activity than 60% ethanol extract of dark tea in the concentration 25μg/mL, inhibition were 69.31%, 77.53% and 75.23%, respectively. Fraction D -7 was selected to further separation by HSCCC with solvent system ethyl acetate-n-buthanol-methanol-water-acetic acid (4:1:1:4:0.1 ,v/v). Compound 1 (vitexin) and compound 2 (isovitexin) were obtained by one step HSCCC separation with purities higher than 95%, respectively.关键词
黑茶/白叶单枞/α-葡萄糖苷酶/高速逆流色谱Key words
dark tea/ baiye dancong/a-glycosidase/ HSCCC分类
轻工纺织引用本文复制引用
叶琼仙,尹胜,周盈利,苗爱清,李绮汶,刘静,朱龙平,LESLIE Brown,王冬梅..白叶单枞黑茶降血糖活性成分的高速逆流色谱分离[J].食品工业科技,2013,34(6):85-87,107,4.基金项目
广东省自然科学基金(9151064001000038) （9151064001000038）
广东省大学生创新性实验计划课题(1055811179) （1055811179）
中山大学医科学生业余科研基金项目([2012]83号) （[2012]83号）
