食品工业科技2013，Vol.34Issue(6)：236-241,261,7.
碎米双酶解成注射用葡萄糖的液化糖化工艺研究
Study on the liquefied and saccharified craft for the dual-enzyme hydrolysis method of making injection-glucose with broken rice
摘要
Abstract
Objective:To study on the liquefied and saccharified craft for the dual-enzyme hydrolysis method of making injection-glucose with broken rice, and to explore the best process conditions suitable for the preparation of the liquefaction and saccharification. Methods:Used double enzyme method,and mathematical model of Michaelis-Menten equation. Chose the value of DE,the protein content,and the light transmission rate and other indicators. Results:The best grinding broken head was 60～80 mesh. Optimum amount of liquid enzyme 160U·g-1. Liquefaction process for the best pieces rice was to 60 mesh,mixing the concentration was 25% to 30%,liquefaction time was 30min,CaCI2 amount was 0.3%,pH was 6.0,temperature was 90～95℃,Hong Ying Xiang enzyme dosage was 160U/g. Optimum saccharification Hong Ying Xiang glucoamylase for the amount was 150U/g dry matter, pullulanase dosage was 0.25U/g dry matter,saccharification time was 24h,pH of 4.2, the temperature was 60℃. Conclusion:Through this study,the best craft for the liquefaction and saccharification process conditions with the broken rice was solved, and the foundation of making glucose injection with broken rice was set.关键词
碎米/注射用葡萄糖/液化工艺/糖化工艺Key words
broken rice/ glucose for injection/ liquefaction technology/ saccharification technology分类
轻工纺织引用本文复制引用
刘文龙,贺福元,杨岩涛,石继连,邹欢,唐昱,邱云,黄胜,陈锋..碎米双酶解成注射用葡萄糖的液化糖化工艺研究[J].食品工业科技,2013,34(6):236-241,261,7.基金项目
湖南省科技厅项目(2010SK3019) （2010SK3019）
国家自然科学基金青年基金项目(30901971) （30901971）
国家自然科学基金连续面上项目(81270055). （81270055）
