食品与发酵工业2013，Vol.39Issue(1)：6-10,5.
高产琥珀酸重组大肠杆菌的构建及厌氧发酵
Construction and anaerobic fermentation of genetically engineered Escherichia coli for the production of succinic acid
摘要
Abstract
In this study, metabolic pathway engineering using Red recombinase system was performed on Escherichia coli W, a wild type strain, for the production of succinic acid. In order to eliminate the by-product formation of E. coli fermentation, five genes leading for production of lactate (ldhA) , formate. (pflB) ethanol (adhE) , and acetate (poxB and ackA) were deleted from the chromosome. The resulting mutant was then further selected through a-daptive (metabolic) evolution for improved anaerobic growth. The evolved strain WS100 could produce succinate as the major fermentation product. In a 15 L fermentation under anaerobic condition using mineral salt medium, WS100 produced 70. 13 g/L succinate from 100 g/L glucose in 72 h, with a volumetric productivity of 0. 98 g/( L · h) and a yield of 76% based on sugar metabolism. Although small amount of acetate and lactate were still produced as the minor by - products, there was no detectable production of formate and ethanol. This result demonstrated the great potential of the engineered E. coli WS100 for fermentative production of succinic acid at large scale.关键词
大肠杆菌工程菌/琥珀酸/基因敲除/厌氧发酵Key words
genetically engineered Escherichia coli, succinate, gene deletion, anaerobic fermentation引用本文复制引用
赵锦芳,华渤文,王永泽,赵筱,王金华..高产琥珀酸重组大肠杆菌的构建及厌氧发酵[J].食品与发酵工业,2013,39(1):6-10,5.基金项目
国家自然科学基金项目(NSFC31070094) （NSFC31070094）
湖北省科技厅科研项目(2011CDA008) （2011CDA008）
湖北省教育厅优秀中青年人才项目(Q20121405) （Q20121405）
湖北省楚天学者专项基金 （）
