食品与发酵工业2013，Vol.39Issue(1)：146-150,5.
烧酒曲中扣囊复膜酵母的分离及鉴定
Isolation and identification of Saccharomycopsis fibuligera isolated from rice wine starter
摘要
Abstract
Yeast strain YW12 with amylolytic activity was isolated from rice wine solid starter diluted and spread on starch-degrading plate ( YPS agar) to make pure culture starter for rice wine. Based on morphological, cultural and physiological tests and phylogenetic analysis of 18S rDNA and internal transcribed spacer (ITS) sequences, YW12 was identified as Saccharomycopsis fibuligera. Detected with the modified Yoo method, amylase activity of crude enzyme solution from YW12 in YPS broth for 4 days at 28 ℃ was 49. 8 U/mL. The strain YW12 cultured in saccharification mash with 170 g/L glucose from glutinous rice for 3 days at 28 ℃ gave 5. 63 % ( v/v) of ethanol content. Because of its ability of starch-degrading and ethanol fermentation, the strain YW12 maybe have a potential to be used in making defined fungal starter for glutinous rice wine.关键词
扣囊复膜酵母/淀粉酶/18S rDNA/ITS区Key words
Saccharomycopsis fibuligera, amylase, 18S rDNA, ITS region引用本文复制引用
应玲云,伍时华,赵东玲,何启刚,刘光鹏,黄翠姬,易弋..烧酒曲中扣囊复膜酵母的分离及鉴定[J].食品与发酵工业,2013,39(1):146-150,5.基金项目
广西科技厅项目(桂科攻0782003-2) （桂科攻0782003-2）
柳州市科技局项目(2007021102) （2007021102）
