食品与发酵工业2013，Vol.39Issue(1)：181-186,6.
液相色谱-电喷雾飞行时间质谱法同时测定虫生真菌菌丝中16种游离氨基酸
Simultaneous analysis of 16 free amino acids in mycelia of entomogeneous fungi by LC-ESI-TOF-MS
摘要
Abstract
A method for simultaneous analysis of 16 free amino acids in entomogeneous fungi was developed using liquid chromatography-electrospray time-of-flight mass spectrometry ( LC-ESI-TOF-MS) under negative ionization mode. The results showed that chromatographies and ion features of the 16 amino acids had obvious characteristics which can be used for accurately qualitative analysis. Quantitative tests showed that the 16 amino acids had wide linear ranges and high accuracy. In the linear range from 4. 0 to 125μmol/L all the correlation coefficients between concentration and peak area of the 16 amino acid were above 0. 998. At the concentrations of 50μmol/L the relative standard deviations of the 16 amino acids were in the range from 0. 84% to 2. 75% . Results of LC-ESI-TOF-MS analysis of the 16 free amino acids in mycelia of 7 kinds of entomogenous fungi showed that the contents of the 16 amino acids were very different in the same strain. Of all the amino acids the content of phenylalanine was the highest one in all tested strains, and its range was from 0.718 to 2. 173 mg/g. Hirsutella sinensis contained the highest phenylalanine. Apart from phenylalanine the contents of proline, valine, leucine, isoleucine, lysine and glutamic acid were also obviously higher than the other amino acids, and most of them were higher than 0. 3mg/g. The amino acids with relative higher contents could be used as characteristic index for identification of free amino acids in entomogenous fungi. The total free amino acids contents in mycelia of different strains were also significantly different. The content of Hirsutella sinensis was 7. 537mg/g which was 1. 36 times higher than that of Isaria cicadae (3. 196mg/g).关键词
液相色谱-质谱联用/游离氨基酸/虫生真菌Key words
liquid chromatography-mass spectrometry (LC-MS) ,free amino acids, entomogeneous fungi引用本文复制引用
刘肖肖,李淑林,陆瑞利,罗飞飞,胡丰林..液相色谱-电喷雾飞行时间质谱法同时测定虫生真菌菌丝中16种游离氨基酸[J].食品与发酵工业,2013,39(1):181-186,6.基金项目
国家自然科学基金项目(30871676) （30871676）
国家863计划项目(2007AA021506) （2007AA021506）
