现代食品科技2013，Vol.29Issue(4)：768-771,4.
阴离子交换层析法分离纯化花生主要过敏原Ara h1的研究
Purification of Peanut Allergen Ara h1 with Anion-exchange Chromatography
摘要
Abstract
Protien Ara hi is a major allergen in peanut In order to obtain the natural peanut allergen Ara hi, ammonium sulfate precipitation and anion-exchange chromatography were used for purification. The analysis and identification of peanut allergens was carried out using SDS-PAGE and Western blot The results showed that the purity of Ara hi was more than 90 percent and the recovery accounted 23.1% of total Ara hi with the anion-exchange chromatography. The Ara hi purfied by anion-exchange chromatography had immunocompetence. It can combine to peanut allergy patients serum with high specificity.关键词
花生/食物过敏/纯化/阴离子交换层析Key words
peanut/ food allergy/ purification/ anion-exchange chromatography引用本文复制引用
何伟逸,吴序栎,刘志刚,黄海珍,李瑶,叶烨,曹鹤瑶..阴离子交换层析法分离纯化花生主要过敏原Ara h1的研究[J].现代食品科技,2013,29(4):768-771,4.基金项目
国家自然科学基金项目(31101280) （31101280）
广东省自然科学基金(S2012010008514) （S2012010008514）
深圳市重点实验室项目(SW201110010) （SW201110010）
