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高产淀粉酶和蛋白酶的黑曲霉菌株的发酵条件优化

谭海刚 李静 杨文浩

现代食品科技2013,Vol.29Issue(4):808-811,4.
现代食品科技2013,Vol.29Issue(4):808-811,4.

高产淀粉酶和蛋白酶的黑曲霉菌株的发酵条件优化

Optimization of Fermentation Conditions for the Produciton of Amylase and Protease by a Strain of Aspergillus niger

谭海刚 1李静 1杨文浩1

作者信息

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摘要

Abstract

A strain A020 of Aspergillus niger with high productivity of amylase and protease was isolated. The optimum compositions of fermentation medium were as followed: 1% peptone, 1% maltose and 3×10-3 mol/L NaCl in banana peel basic medium. And the optimum fermentation conditions were achieved as follows: temperature 30 ℃, initial pH 6.5 and medium volume 150 mL in 500 mL flask. Under these conditions, the activities of amylase and protease and cultured for 4 d at 150 r/min were 1278 U/mL and 918 U/mL, respectively. The amylase and protease activities increased by 68.2% and 41.2%, respectively.

关键词

黑曲霉/蛋白酶/淀粉酶

Key words

Aspergillus niger, protease/ amylase

引用本文复制引用

谭海刚,李静,杨文浩..高产淀粉酶和蛋白酶的黑曲霉菌株的发酵条件优化[J].现代食品科技,2013,29(4):808-811,4.

基金项目

青岛农业大学第四届大学生创新教育立项(2010) (2010)

现代食品科技

OA北大核心CSTPCD

1673-9078

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