现代食品科技2013，Vol.29Issue(4)：894-897,4.
反相高效液相色谱法测定烟草中的茄尼醇
Determination of Solanesol in Tobacco by RP-HPLC
摘要
Abstract
A simple and rapid method was developed for determination of solanesol in tobacco by RP-HPLC and the saponification process was optimized. The solanesol content was quantitatively determined with the external standard method. The results indicated that the optimum saponification process was as follows: KOH -methanol solution 0.5 mol/L, of saponification temperature 30 ℃, and the saponification time 30 minutes. The method had good linearity with correlation coefficient of 0.9999. The limit of detection based on signal-to-noise ratio of 3 was 27.4 ng. The recovery of solanesol in tobacco was 99% with RSD of 0.41%.关键词
茄尼醇/反相高效液相色谱/烟草Key words
solanesol/ RP-HPLC/ tobacco引用本文复制引用
舒俊生,陈开波,徐志强,毛健..反相高效液相色谱法测定烟草中的茄尼醇[J].现代食品科技,2013,29(4):894-897,4.
