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超声强化大豆分离蛋白-糖接枝反应作用机理的初步探讨

穆利霞 赵谋明 颜小平 廖森泰

食品工业科技2013,Vol.34Issue(7):90-95,6.
食品工业科技2013,Vol.34Issue(7):90-95,6.

超声强化大豆分离蛋白-糖接枝反应作用机理的初步探讨

Preliminary discussion on mechanism of ultrasonic enhanced soy protein isolates-saccharide graft reaction

穆利霞 1赵谋明 1颜小平 1廖森泰1

作者信息

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摘要

Abstract

The mechanism of ultrasound promoting soy protein isolate(SPI)-saccharide graft reaction was studied by using soybean protein isolates as the research object.Compared with classical wet-heating, content of free amino acid increased from 0.86mol/L to 0.98mol/L when SPI was treated by ultrasonic for 60min. Surface hydrophobicity and content of apparent sulfhydryl content were also increased to a certain extent respectively. In this case,polypeptide chains of protein were more stretch,and part of free amino groups buried in the molecules inside were exposed.At the same time,α-helix and β-folding of SPI was significantly reduced while irregular coil was significantly increased,that showed ordered molecule structures become disordered and its steric hindrance reduced.The graft reaction of SPI-saccharide was accelerated because their more contacting led by intense vibration of ultrasound.

关键词

超声处理/大豆分离蛋白(SPI)/接枝反应/机制

Key words

ultrasonic treatment/ soy protein isolates (SPI)/ graft reaction/ mechanism

分类

轻工纺织

引用本文复制引用

穆利霞,赵谋明,颜小平,廖森泰..超声强化大豆分离蛋白-糖接枝反应作用机理的初步探讨[J].食品工业科技,2013,34(7):90-95,6.

基金项目

现代农业产业技术体系建设专项资金财教[2008]370号. ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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