食品工业科技2013，Vol.34Issue(7)：102-105,4.
动态高压微射流诱导的去折叠胰蛋白酶结构预测
Prediction and simulating of the conformation of unfolded trypsin induced by dynamic high-pressure microfluidization
摘要
Abstract
Differential scanning calorimetry and circular dichroism were used to analyze the conformational change of trypsin induced by dynamic high-pressure microfluidization(DHPM).Based on the reflection in conformational change of the unfolding trypsin induced by DHPM in our previous study,the conformation of native and unfolding trypsin was predicted and modeled by PyMOL and 3DS Max. The results indicated that compared with native trypsin,the decrease in thermal enthalpy and the change of percentages of secondary structural elements, the loose structure of unfolding trypsin was found. The active center, disulfide bond and the secondary structural information of trypsin was visually viewed by the modeled conformation figure.In addition,preliminary study on the structural modeling change of DHPM-induced unfolding trypsin was completed.关键词
胰蛋白酶/动态高压微射流/去折叠/结构预测Key words
trypsin/dynamic high-pressure microfluidization( DHPM)/unfolding/conformation prediction分类
轻工纺织引用本文复制引用
张兆琴,刘伟,刘军平,邹立强,刘成梅..动态高压微射流诱导的去折叠胰蛋白酶结构预测[J].食品工业科技,2013,34(7):102-105,4.基金项目
国家自然科学基金(31060209) （31060209）
国家重点实验室重点青年基金(SKLF-QN-201101) （SKLF-QN-201101）
江西省青年科学家培养对象计划(20112BCB23003). （20112BCB23003）
