食品工业科技2013，Vol.34Issue(7)：169-172,177,5.
响应面优化酶法制备茶叶蛋白抗氧化肽的工艺研究
Optimization of preparing antioxidant peptides by enzymatic hydrolysis of tea protein by response surface methodology
摘要
Abstract
Tea residue was used as raw material to produce antioxidant peptides by Alcalase2.4L,the antioxidant activity of hydrolysates was evaluated by reducing power.The influences of the five process parameters which were substrate concentration,enzyme concentration, temperature, pH and enzymatic hydrolysis time were studied.The response surface methodology were used to optimize the enzymatic hydrolysis process. The results showed that the optimum conditions were substrate concentration 3.3%, enzyme concentration 1.8% .temperature 51℃,pH9 and time of enzymatic hydrolysis 2.5h.Under these conditions,the highest reducing power of hydrolysates could be obtained.关键词
茶渣/碱性蛋白酶/抗氧化肽/还原能力/响应面分析Key words
tea residue/Alcalase2.4L/antioxidant peptides/reducing capacity/response surface methodology分类
轻工纺织引用本文复制引用
毛银,朱科学,张士康,王彬,周惠明..响应面优化酶法制备茶叶蛋白抗氧化肽的工艺研究[J].食品工业科技,2013,34(7):169-172,177,5.基金项目
国家"十二五"科技支撑计划课题(2012BAD36B06). （2012BAD36B06）
