食品工业科技2013，Vol.34Issue(7)：309-313,318,6.
HPLC-ELSD同时分离检测食品中十种单糖、双糖和低聚果糖
Simultaneous determination and separation of ten monosaccharides,disaccharides and Fructooligosaccharide by HPLC-ELSD
摘要
Abstract
In order to manage the simultaneous separation and detection of ten sugars (xylose, fructose, glucose, galactose,sucrose,maltose,lactose, 1-kestose, nystose, 1F-Fructofuranosyl nystose)in foods,a separation and detection method was built,which was separated by NH, column with gradient elution of acetonitrile-water as the mobile phase, detected by ELSD. Each sugar had a good linear within 0.1~5mg/mL,the linear correlation coefficients were between 0.9901~0.9996,the detection limits were all less than 0.1mg/mL.The values of RSD were in the range of 1.71%~7.21% and the recovery rate ranged from 97.91%~103.35%.The results showed that this method was efficient,simple and fast and could be used as the simultaneous determination and separation method for these sugars in foods.关键词
单糖/双糖/低聚果糖/高效液相色谱(HPLC)/蒸发光散射检测器(ELSD)Key words
monosaccharide/ disaccharide/ FOS/ HPLC/ ELSD分类
农业科技引用本文复制引用
李灿,陈英,李培,钱和,姚卫蓉..HPLC-ELSD同时分离检测食品中十种单糖、双糖和低聚果糖[J].食品工业科技,2013,34(7):309-313,318,6.基金项目
国家质检总局科技计划项目(2009QK147). （2009QK147）
