食品工业科技2013，Vol.34Issue(7)：325-329,364,6.
基于荧光法的牛奶中单诺沙星的快速检测
Rapid determination of danofloxacin in milk based on fluorescence spectroscopy
摘要
Abstract
The aim of this paper was to develop a rapid screening method to determine danofloxacin( DANO) in milk by fluorescence spectroscopy combining with three different chemometric tools. The fluorescence spectra of 27 whole milk samples from a commercial brand spiked with different concentrations of DANO (0~56μg/L) were recorded as the calibration set after deproteinization.Qualitative and quantitative calibration models were built by PLSDA and PLS,and tested by prediction sets, which were spiked with DANO using UHT and pasteurized milk samples from 4 commercial brands. It was found that PCA could characterize the samples with various concentrations of DANO and PLSDA model and classify milk samples as compliant or non-compliant accurately. The results predicted by PLS model showed that recovery percentage of prediction samples was about 88% ~ 114.4% and RMSEP was during acceptable limits.关键词
荧光法/牛奶/单诺沙星/化学计量学Key words
fluorescence spectroscopy/ milk/ DANO/chemometrics分类
轻工纺织引用本文复制引用
冯仕云,张燕萍,张秋香,刘小鸣,张灏,陈卫..基于荧光法的牛奶中单诺沙星的快速检测[J].食品工业科技,2013,34(7):325-329,364,6.基金项目
国家自然科学基金项目(30901128). （30901128）
