食品工业科技2013，Vol.34Issue(7)：374-377,4.
Taqman荧光探针技术在食源性致病菌检测中的应用
Application of Taqman-based Real-time PCR for detection genes of food-borne pathogens
摘要
Abstract
As the representation of quantitative PCR method, Taqman - based real-time PCR which is the combination of PCR and fluorescent labeling technology,get good specificity and sensitivity by primers and probe dual control the selection of the target gene.Recently,this technique was introduced and applied in detection food -born pathogen.This paper summarized the principle and advantages and disadvantages of Taqman-based real -time PCR.Furthermore the applications of Taqman-based real-time PCR in in detection food-born pathogen were reviewed.The trends of Taqman-based real-time PCR techniques were also discussed in this paper.关键词
Taqman探针/食源性致病菌/定量检测Key words
Taqman probe/food-born pathogen/quantitative detection分类
轻工纺织引用本文复制引用
伊鋆,蔡雪凤..Taqman荧光探针技术在食源性致病菌检测中的应用[J].食品工业科技,2013,34(7):374-377,4.基金项目
国家质检总局科研课题(2011IKIK192). （2011IKIK192）
