食品与发酵工业2012，Vol.38Issue(12)：74-77,4.
高通量筛选葡萄糖酸钙高产菌株
Studies on High Throughput Screening for Calcium Gluconate High Producing Strains
摘要
Abstract
Using Aspergillus niger as the original strain, a high throughput method was established to screen calcium gluconate high producing strains with 48-well plates. From the spores dealed with UV-LiCl complex mutation, 4 high-product strains was obtained by high throughput screening, whose fermentation efficiency was excellent after the passages of strains. According to high product of 23. 04 g/100 mL for the strain of P-9, the yield was increased by 5. 86 g/mL. Comparing with the traditional shake flask screening platform, this method could significantly improve the screening efficiency of calcium gluconate high producing strains.关键词
葡萄糖酸钙/黑曲霉/复合诱变/48孔板/高通量筛选Key words
calcium gluconate/Aspergillus niger/complex mutation/48-well plate/high-throughput screening引用本文复制引用
冯芳,赵伟,孙姗姗..高通量筛选葡萄糖酸钙高产菌株[J].食品与发酵工业,2012,38(12):74-77,4.
