食品与发酵工业2012，Vol.38Issue(12)：138-142,5.
甲硫氨酸氨肽酶高通量微型化检测方法的建立
Establishment of a High Throughput Method for Detecting Methionine Aminopeptidase Activity
摘要
Abstract
The substrate methionine p-nitroaniline in this paper had been used to measure methionine aminopeptidase activity. The accuracy and sensitivity of the detection were improved and the enzyme panel method was established baseed on the optimization of determination conditions, to adapt to the detection for miniaturization fermentation and the high throughput screening for high aminopeptidase-producing strains. The optimal determination conditions were as follows; 40 mmol/L ammonium chloride/ammonia buffer, pH 8. 0, 6 mmol/L methionine p-nitroaniline, reaction temperature 60℃ , reaction time 10 min, measured colormetrically at 380 nm.关键词
氨肽酶/甲硫氨酸氨肽酶/高通量筛选Key words
aminopeptidase/methionine aminopeptidase/high throughput screening引用本文复制引用
杨玉兰,姜文侠,刘琦,孙瑞雪..甲硫氨酸氨肽酶高通量微型化检测方法的建立[J].食品与发酵工业,2012,38(12):138-142,5.基金项目
国家"十二五"863计划课题"啤酒酵母与果酒废渣资源化利用关键技术"(2012AA021403)和天津市滨海新区科技小巨人成长计划项目"食品酶工程关键技术与应用开发平台建设"(2011-XJR12066)资助 （2012AA021403）
