食品与发酵工业2012，Vol.38Issue(12)：157-160,4.
超高效液相色谱串联质谱法快速测定乳及乳制品中三聚氰胺
Rapid Determination of Melamine Residue in Milk and Milk Products by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
胡强 1王延云 1李超豪 1吴卫 1向乐曦 1杨春林1
作者信息
摘要
Abstract
A rapid and economic ultra performance liquid chromatography-tandem mass spectrometry method is developed and validated for the determination of melamine in milk and milk products. The method includes a simple acetonitrile-water extraction, and ultrasound treatment and dichloromethane liquid-liquid extraction to remove protein and fat. The purified extract is further diluted before injection on ACQUITY UPLC BEH HILIC. A positive ion elec-trospray ionization ( ESI+) and multiple reaction monitoring mode (MRM) are then applied. The samples are quantified by external standard method. A total analysis was done within 1. 2 min with a good linear relationship ( r ＞ 0.9991). The limit of detection and the limit of quantification were 0.005 andO. 017 mg/kg, respectively. The spiked recoveries are within 91. 3% -97. 1 % at the spiked levels of 10 -400 μg/L for melamine with relative standard deviation (RSD) below 9.03%. This method is rapid, economic, accurate and sensitive, suitable for screening and detection melamine in milk and milk products.关键词
超高效液相色谱-串联质谱/快速/乳及乳制品/三聚氰胺Key words
ultra performance liquid chromatography-tandem mass spectrometry/rapid/milk and milk products/melamine引用本文复制引用
胡强,王延云,李超豪,吴卫,向乐曦,杨春林..超高效液相色谱串联质谱法快速测定乳及乳制品中三聚氰胺[J].食品与发酵工业,2012,38(12):157-160,4.
