食品与机械2013，Vol.29Issue(2)：60-63,4.DOI:10.3969/j.issn.1003-5788.2013.02.013
PCR-DGGE法分析婴儿肠道菌群多样性
Diversity analysis of infant intestinal microflora by using PCR-DGGE
摘要
Abstract
Purpose: The diversity of the infant intestinal microflora was analyzed by using the PCR-DGGE, providing theoretical basis for the application of probiotics in infant formula milk powder. Method: The universal primer of the bacterial 16S rDNA-V3 region was used as primer and the total DNA of food-bome diarrhea infants and health infants stool samples were used as a template. After PCR amplification, DGGE analysis was performed. Result: The DGGE profiles of the 30 stool samples were highly polymorphic. The diversity of the food-borne diarrhea infants was significantly different with healthy infants, and the Food-bome diarrhea infants had a lower intestinal diversity. By cluster analysis and similarity analysis, Can be drawn that the similarity of intestinal microflora of food-borne diarrhea infants was higher, and the intestinal microflora of food-bome diarrhea infants and healthy infants had obvious differences. Conclusion: PCR-DGGE technology can preliminary analyze the diversity of the infant intestinal microflora, providing theoretical basis for the application of probiotics in infant formula milk powder.关键词
婴儿配方乳粉/16S rDNA/PCR-GGE/肠道菌群多样性Key words
infant formula milk powder/ 16S rDNA/ PCR-DGGE/ intestinal microflora diversity引用本文复制引用
费鹏,白洪健,程述震,曹飞扬,郭鸰,姜亦超,苑秀娟,江岩..PCR-DGGE法分析婴儿肠道菌群多样性[J].食品与机械,2013,29(2):60-63,4.基金项目
教育部创新团队发展计划(编号:IRT-0059-303) （编号:IRT-0059-303）
黑龙江省博士后科研启动金(编号:LBH-Q12149) （编号:LBH-Q12149）
